Abstract
The enantioselectivity of a Burkholderia cepacia lipase toward secondary alcohols could be both increased and decreased rationally by introducing only a single mutation on the basis of the mechanism proposed previously.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alcohols / chemistry
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Burkholderia cepacia / enzymology
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DNA, Bacterial / genetics
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Escherichia coli / genetics
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Lipase / genetics*
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Lipase / metabolism*
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Models, Molecular
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Mutagenesis, Site-Directed*
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Reverse Transcriptase Polymerase Chain Reaction
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Stereoisomerism
Substances
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Alcohols
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DNA, Bacterial
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Lipase