Rational control of enantioselectivity of lipase by site-directed mutagenesis based on the mechanism

Chem Commun (Camb). 2005 Oct 7:(37):4650-1. doi: 10.1039/b508244g. Epub 2005 Aug 12.

Abstract

The enantioselectivity of a Burkholderia cepacia lipase toward secondary alcohols could be both increased and decreased rationally by introducing only a single mutation on the basis of the mechanism proposed previously.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohols / chemistry
  • Burkholderia cepacia / enzymology
  • DNA, Bacterial / genetics
  • Escherichia coli / genetics
  • Lipase / genetics*
  • Lipase / metabolism*
  • Models, Molecular
  • Mutagenesis, Site-Directed*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stereoisomerism

Substances

  • Alcohols
  • DNA, Bacterial
  • Lipase