In this paper, we report a novel technique for the manufacture of polymeric barcoded strips having diverse characteristics such as biocatalyst-based sensing and pH-responsive actuation. The fabrication involves the use of both a microfluidic platform and in-situ photopolymerization. It is expected that this method, which is a very simple, cost-effective, and environment-friendly means for mass production, will facilitate the stable immobilization of diverse biological substances such as enzymes, DNA, and antigens. Therefore, we fabricated an enzyme-immobilized barcoded strip for multiple bio-analysis. On the basis of this technology, we adopted different materials and thereby fabricated programmable microactuation components that are responsive to pH variation.