Assessing phylogenetic relationships of Lycium samples using RAPD and entropy theory

Acta Pharmacol Sin. 2005 Oct;26(10):1217-24. doi: 10.1111/j.1745-7254.2005.00197.x.

Abstract

Aim: To evaluate the phylogenetic relationships among related species of Lycium samples.

Methods: Random amplified polymorphic DNA (RAPD) fingerprinting and lab-on-a-chip electrophoresis techniques were used to analyze the characteristics of Lycium species. Seven species and 3 varieties of Lycium were studied. Based on RAPD fingerprint data obtained from 11 primers, we proposed a new index, called dispersivity, using entropy theory and projection methods to depict the diversity of the DNA fingerprints.

Results: Using the proposed dispersivity, primers were sorted and the dendrograms of the 7 species and 3 varieties of Lycium were constructed synthetically by merging primer information.

Conclusion: Phylogenetic relationships among Lycium samples were constructed synthetically based on RAPD fingerprint data generated from 11 primers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Fingerprinting
  • DNA Primers / genetics
  • DNA, Plant / genetics
  • Entropy
  • Genetic Variation
  • Lycium / classification
  • Lycium / genetics*
  • Microchip Analytical Procedures
  • Phylogeny*
  • Plants, Medicinal / classification
  • Plants, Medicinal / genetics*
  • Random Amplified Polymorphic DNA Technique / methods

Substances

  • DNA Primers
  • DNA, Plant