Multiplex polymerase chain reaction and ligation detection reaction/universal array technology for the traceability of genetically modified organisms in foods

C Peano; R Bordoni; M Gulli; A Mezzelani; M C Samson; G De Bellis; N Marmiroli

doi:10.1016/j.ab.2005.08.004

Multiplex polymerase chain reaction and ligation detection reaction/universal array technology for the traceability of genetically modified organisms in foods

Anal Biochem. 2005 Nov 1;346(1):90-100. doi: 10.1016/j.ab.2005.08.004. Epub 2005 Aug 25.

Authors

C Peano¹, R Bordoni, M Gulli, A Mezzelani, M C Samson, G De Bellis, N Marmiroli

Affiliation

¹ Institute of Biomedical Technologies, National Research Council, Via Fratelli Cervi 93, Segrate, Milano 20090, Italy. clelia.peano@itb.cnr.it

PMID: 16169511
DOI: 10.1016/j.ab.2005.08.004

Abstract

A multiplex polymerase chain reaction (PCR) system was developed for the simultaneous detection of target sequences in genetically modified soybean (Roundup Ready) and maize (MON810, Bt176, Bt11, and GA21). Primer pairs were designed to amplify the junction regions of the transgenic constructs analyzed and the endogenous genes of soybean (lectin) and maize (zein) were included as internal control targets to assess the efficiency of all reactions. This multiplex PCR has constituted the basis for an efficient platform for genetically modified organism traceability based on microarray technology. In particular, the ligation detection reaction combined to a universal array approach, using the multiplex PCR as target, was applied. High specificity and sensitivity were obtained.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA, Plant / analysis*
Food Analysis*
Food, Genetically Modified*
Glycine max / genetics*
Oligonucleotide Array Sequence Analysis
Polymerase Chain Reaction / methods*
Zea mays / genetics*

Substances

DNA, Plant