Specific photodissociation of peptides with multi-stage mass spectrometry

Rapid Commun Mass Spectrom. 2005;19(20):2883-92. doi: 10.1002/rcm.2124.

Abstract

We report collision-induced dissociation (CID) and laser-induced dissociation (LID) performed at different wavelengths between 220 and 280 nm of the peptides leucine-enkephalin (protonated) and gramicidin A (sodiated). Hydrogen-atom losses and side-chain cleavages were observed in LID experiments. These losses depend on the laser wavelength and lead to the formation of radical ions. The fragmentations of these radicals, which are not observed in CID experiments, were investigated in multi-stage mass spectrometry experiments.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Enkephalin, Leucine / analysis*
  • Enkephalin, Leucine / radiation effects*
  • Equipment Design
  • Equipment Failure Analysis
  • Gramicidin / analysis*
  • Gramicidin / radiation effects*
  • Lasers
  • Peptide Mapping / instrumentation
  • Peptide Mapping / methods*
  • Peptides / analysis
  • Peptides / radiation effects
  • Photochemistry / methods*
  • Spectrometry, Mass, Electrospray Ionization / instrumentation
  • Spectrometry, Mass, Electrospray Ionization / methods*
  • Ultraviolet Rays

Substances

  • Peptides
  • Gramicidin
  • Enkephalin, Leucine