Abstract
An Escherichia coli K-12 strain was constructed with a chromosomal deletion (mutSdelta800) in the mutS gene that produced the removal of the C-terminal 53 amino acids which are not present in the MutS crystal structure. This strain has a MutS null phenotype for mutation avoidance, anti-recombination, and sensitivity to cytotoxic agents in a dam mutant background.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphatases / chemistry
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Adenosine Triphosphatases / metabolism*
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Bacterial Proteins / chemistry
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Bacterial Proteins / metabolism*
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Base Pair Mismatch
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DNA Repair / physiology*
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / metabolism*
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Escherichia coli / genetics*
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Escherichia coli / physiology
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Escherichia coli Proteins*
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MutS DNA Mismatch-Binding Protein
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Mutation*
Substances
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Bacterial Proteins
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DNA-Binding Proteins
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Escherichia coli Proteins
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Adenosine Triphosphatases
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MutS DNA Mismatch-Binding Protein
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MutS protein, E coli