Hairy root cultures of Gynostemma pentaphyllum (Thunb.) Makino: a promising approach for the production of gypenosides as an alternative of ginseng saponins

Biotechnol Lett. 2005 Aug;27(16):1165-9. doi: 10.1007/s10529-005-8653-7.

Abstract

Hairy root cultures of Gynostemma pentaphyllum were established by infecting leaf discs with Agrobacterium rhizogenes. The dry biomass of hairy roots grown in MS medium for 49 days was 7.3 g l(-1) with a gypenoside content of 38 mg g(-1) dry wt.

MeSH terms

  • Cloning, Molecular / methods*
  • Gynostemma / genetics*
  • Gynostemma / metabolism*
  • Panax / metabolism
  • Plant Extracts / biosynthesis
  • Plant Roots / genetics*
  • Plant Roots / metabolism*
  • Plants, Genetically Modified / metabolism*
  • Recombinant Proteins / biosynthesis
  • Saponins / biosynthesis
  • Transfection / methods*

Substances

  • Plant Extracts
  • Recombinant Proteins
  • Saponins
  • gypenoside