Secretion of recombinant pediocin PA-1 by Bifidobacterium longum, using the signal sequence for bifidobacterial alpha-amylase

Appl Environ Microbiol. 2005 Sep;71(9):5630-2. doi: 10.1128/AEM.71.9.5630-5632.2005.

Abstract

A recombinant DNA, encoding the chimeric protein of the signal sequence for bifidobacterial alpha-amylase mature pediocin PA-1, was introduced into Bifidobacterium longum MG1. Biologically active pediocin PA-1 was successfully secreted from the strain and showed bactericidal activity against Listeria monocytogenes and the same molecular mass as native pediocin PA-1.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriocins / genetics
  • Bacteriocins / metabolism*
  • Bacteriocins / pharmacology
  • Bifidobacterium / genetics
  • Bifidobacterium / metabolism*
  • Gene Expression Regulation, Bacterial
  • Listeria monocytogenes / drug effects*
  • Pediocins
  • Protein Sorting Signals*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism*
  • alpha-Amylases / genetics
  • alpha-Amylases / metabolism

Substances

  • Bacteriocins
  • Pediocins
  • Protein Sorting Signals
  • Recombinant Proteins
  • pediocin PA-1
  • alpha-Amylases