7-hydroxy-3-(4-hydroxybenzyl)chroman and broussonin b: neurotrophic compounds, isolated from Anemarrhena asphodeloides BUNGE, function as proteasome inhibitors

Biol Pharm Bull. 2005 Sep;28(9):1798-800. doi: 10.1248/bpb.28.1798.

Abstract

The extract of Anemarrhenae Rhizoma (rhizomes of Anemarrhena asphodeloides BUNGE) showed neurotrophic activity toward rat pheochromocytoma (PC-12) cells. Bioassay-guided purification afforded four compounds, 2,6,4'-trihydroxy-4-methoxybenzophenone (1), 7-hydroxy-3-(4-hydroxybenzyl)chroman (2), broussonin B (3), and cis-hinokiresinol (4). Compounds 1-3 induced neurite outgrowth in PC-12 cells at concentration of 50 microg/ml, while 4 was less active. In addition, compounds 2-4 showed moderate inhibitory activities against a chymotrypsin-like activity of the proteasome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkanes / pharmacology*
  • Anemarrhena / chemistry*
  • Animals
  • Benzyl Compounds / pharmacology*
  • Central Nervous System Agents / pharmacology*
  • Chromans / pharmacology*
  • Chymotrypsin / chemistry
  • Magnetic Resonance Spectroscopy
  • Nervous System / drug effects*
  • Neurites / drug effects
  • PC12 Cells
  • Phenols / pharmacology*
  • Plant Roots / chemistry
  • Protease Inhibitors / chemistry
  • Protease Inhibitors / isolation & purification
  • Protease Inhibitors / pharmacology*
  • Rats

Substances

  • 7-hydroxy-3-(4-hydroxybenzyl)chroman
  • Alkanes
  • Benzyl Compounds
  • Central Nervous System Agents
  • Chromans
  • Phenols
  • Protease Inhibitors
  • broussonin B
  • Chymotrypsin