[Screen selection of target genes regulated by HSF1 in heat shock response from myocardium of gene knockout mice]

Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2004 Feb;29(1):1-5.
[Article in Chinese]

Abstract

Objective: To screen select target genes regulated by heat shock transcription factor 1 (HSF1 ) in heat shock response from myocardium of HSF1 gene knockout mice using cDNA chip.

Methods: HSF1 knockout mice were subjected to heat shock treatment (42 degrees C for 15 minutes, and recovered for 3 hours), and cDNA chip containing 15 000 mouse cDNAs were employed to investigate the expression of genes regulated by HSF1 in the myocardium. Then 4 of the differentially expressed genes in the myocardium of HSF1 knockout mice were further confirmed by RT-PCR. The promoter regions of the differentially expressed genes were analysed by TESS software to find HSE.

Results: Three hundred and ninety-eight genes were down - regulated in the myocardium of HSF1 knockout mice in heat shock response,with 173 known. Whereas 641 genes were up-regulated in the myocardium, with 235 known. The 5 down-regulated genes and 6 up-regulated ones were shown to contain HSE in their promoter regions.

Conclusion: In heat shock response, expression of multiple genes is regulated directly or indirectly by HSF1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / physiology*
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Heat Shock Transcription Factors
  • Heat-Shock Response / genetics*
  • Male
  • Mice
  • Mice, Knockout
  • Myocardium / metabolism*
  • Oligonucleotide Array Sequence Analysis
  • Transcription Factors / genetics
  • Transcription Factors / physiology*

Substances

  • DNA-Binding Proteins
  • Heat Shock Transcription Factors
  • Transcription Factors