Targeting of the virulence factor acetohydroxyacid synthase by sulfonylureas results in inhibition of intramacrophagic multiplication of Brucella suis

Antimicrob Agents Chemother. 2005 Sep;49(9):3922-5. doi: 10.1128/AAC.49.9.3922-3925.2005.

Abstract

The acetohydroxyacid synthase (AHAS) of Brucella suis can be effectively targeted by the sulfonylureas chlorimuron ethyl and metsulfuron methyl. Growth in minimal medium was inhibited, and multiplication in human macrophages was totally abolished with 100 microM of sulfonylureas. Metsulfuron methyl-resistant mutants showed reduced viability in macrophages and reduced AHAS activity.

MeSH terms

  • Acetolactate Synthase / antagonists & inhibitors*
  • Arylsulfonates / pharmacology
  • Brucella suis / drug effects*
  • Brucella suis / growth & development*
  • Culture Media
  • Drug Delivery Systems
  • Macrophages / microbiology*
  • Sulfonylurea Compounds / pharmacology*
  • Virulence Factors*

Substances

  • Arylsulfonates
  • Culture Media
  • Sulfonylurea Compounds
  • Virulence Factors
  • metsulfuron methyl
  • Acetolactate Synthase