Abstract
The photobiological properties of 6-methoxy-2-naphthylacetic acid (6-MNAA) were studied using a variety of in vitro phototoxicity assays: photohemolysis, photoperoxidation of linoleic acid, photosensitized degradation of histidine and thymine and the Candida phototoxicity test. 6-MNAA was phototoxic in vitro. 6-MNAA reduced nitro blue tetrazolium (NBT) when irradiated with lambda > or = 300 nm in deoxygenated aqueous buffer solution (pH 7.4). NBT can be reduced by reaction with the excited state of 6-MNAA subject to interference with molecular oxygen. The photohemolysis rate was inhibited by the presence of 1,4-diazabicyclo[2.2.2]octane (DABCO), sodium azide (NaN3) and reduced glutathione (GSH). Photoperoxidation of linoleic acid and photosensitized degradation of histidine and thymine were significantly inhibited by sodium azide and reduced glutathione. 6-MNAA was phototoxic to C. albicans, C. lipolytica and C. tropicalis. A mechanism involving singlet oxygen, radicals, and electron transfer reactions is suggested for the observed phototoxicity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aerobiosis
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Anaerobiosis
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Anti-Inflammatory Agents, Non-Steroidal / pharmacokinetics*
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Butanones / pharmacokinetics*
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Candida / drug effects
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Candida / growth & development
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Candida / radiation effects
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DNA / drug effects
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DNA / radiation effects
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Dermatitis, Phototoxic*
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Erythrocytes / drug effects
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Erythrocytes / radiation effects
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Hemolysis / drug effects
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Hemolysis / radiation effects
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Histidine / chemistry
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Histidine / radiation effects
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Humans
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In Vitro Techniques
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Light
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Linoleic Acid / chemistry
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Linoleic Acid / radiation effects
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Lipid Peroxidation / drug effects
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Lipid Peroxidation / radiation effects
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Nabumetone
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Naphthaleneacetic Acids / pharmacology*
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Nitroblue Tetrazolium / chemistry
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Nitroblue Tetrazolium / radiation effects
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Photochemistry
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Photosensitizing Agents / pharmacology*
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Thymine / chemistry
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Thymine / radiation effects
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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Butanones
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Naphthaleneacetic Acids
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Photosensitizing Agents
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Nitroblue Tetrazolium
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Histidine
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DNA
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6-methoxy-2-naphthylacetic acid
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Linoleic Acid
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Nabumetone
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Thymine