Optochemical sensor for an ornidazole assay using 1-amino-4-allyloxyanthraquinone as a fluorescent indicator

Anal Sci. 2005 Aug;21(8):967-71. doi: 10.2116/analsci.21.967.

Abstract

To improve the stability of optochemical sensors (optodes), the fluorescence indicator 1-amino-4-allyloxyanthraquinone (AAA), which was synthesized by reacting allyl bromide with 1-amino-4-hydroxyanthraquinone, was covalently immobilized on surface-modified glass slides. The resulting sensor was used to determine the content of ornidazole based on fluorescence quenching. It showed a linear response toward ornidazole in the concentration range of 9.0 x 10(-6) to 1.0 x 10(-3) mol L(-1) with a detection limit of 8 x 10(-6) mol L(-1) at pH 7.5. This AAA-immobilized sensor has a rapid response, high stability and good selectivity to ornidazole.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anthraquinones / chemistry*
  • Fluorescent Dyes / chemistry
  • Hydrogen-Ion Concentration
  • Nitroimidazoles / chemistry*
  • Optics and Photonics / instrumentation*
  • Ornidazole / chemistry*

Substances

  • Anthraquinones
  • Fluorescent Dyes
  • Nitroimidazoles
  • Ornidazole