Extraction and identification by mass spectrometry of undecaprenyl diphosphate-MurNAc-pentapeptide-GlcNAc from Escherichia coli

Ziqiang Guan; Steven D Breazeale; Christian R H Raetz

doi:10.1016/j.ab.2005.07.002

Extraction and identification by mass spectrometry of undecaprenyl diphosphate-MurNAc-pentapeptide-GlcNAc from Escherichia coli

Anal Biochem. 2005 Oct 15;345(2):336-9. doi: 10.1016/j.ab.2005.07.002.

Authors

Ziqiang Guan¹, Steven D Breazeale, Christian R H Raetz

Affiliation

¹ Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA. zguan@biochem.duke.edu

PMID: 16118008
DOI: 10.1016/j.ab.2005.07.002

Abstract

Undecaprenyl diphosphate-MurNAc-pentapeptide-GlcNAc (lipid II) is extracted from Escherichia coli cells by utilizing its unusual pH-dependent solubility property in a Bligh-Dyer system, and identified by electrospray ionization mass spectrometry in conjunction with a novel 15N mass shift analysis. The described approach will facilitate the structural characterization of lipid II variants from diverse bacteria, including antibiotic-resistant mutants, as well as the numerous minor uncharacterized lipids present in all biological systems.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Diphosphates / chemistry*
Escherichia coli / growth & development
Escherichia coli / metabolism*
Hydrogen-Ion Concentration
Mass Spectrometry*
Muramic Acids / chemistry*
Solubility
Spectrometry, Mass, Electrospray Ionization

Substances

Diphosphates
Muramic Acids

Grants and funding

1U54GM069338/GM/NIGMS NIH HHS/United States