Abstract
A mutant of Escherichia coli enterotoxin induces specific Thl-type T cells to varicella-zoster vaccine. The mutant increased IL-12p40, TNFalpha and nitric oxide production by IFNgamma-stimulated bone marrow macrophages but cholera toxin did not. Anti-TNFalpha antibodies blocked its stimulation of IL-12p40 production but iNOS inhibitor did not. IL-12p40 and IL-12p35 production was stimulated at the level of mRNA formation by the mutant. Cholera toxin suppressed IL-12beta1 expression by spleen T cells stimulated with anti-CD3 antibodies but the mutant did not. These findings indicate that the mutant may induce Thl-type response to the vaccine through its IL-12 and TNFalpha induction by macrophages.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adjuvants, Immunologic / administration & dosage
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Animals
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Bacterial Toxins / administration & dosage
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Bacterial Toxins / genetics
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Bacterial Toxins / immunology*
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Chickenpox Vaccine / administration & dosage
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Chickenpox Vaccine / immunology*
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Cholera Toxin / immunology
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Enterotoxins / administration & dosage
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Enterotoxins / genetics
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Enterotoxins / immunology*
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Escherichia coli Proteins / administration & dosage
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / immunology*
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Escherichia coli*
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Female
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Flow Cytometry
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Gene Expression Regulation
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Herpesvirus 3, Human / immunology
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Interferon-gamma / immunology
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Interleukin-12 / biosynthesis*
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Macrophages / immunology*
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Mutation
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Nitric Oxide / biosynthesis
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RNA, Messenger / analysis
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Receptors, Interleukin / analysis
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Receptors, Interleukin-12
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Th1 Cells / immunology*
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Transcription, Genetic
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Tumor Necrosis Factor-alpha / biosynthesis
Substances
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Adjuvants, Immunologic
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Bacterial Toxins
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Chickenpox Vaccine
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Enterotoxins
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Escherichia coli Proteins
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RNA, Messenger
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Receptors, Interleukin
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Receptors, Interleukin-12
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Tumor Necrosis Factor-alpha
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Interleukin-12
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Nitric Oxide
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Interferon-gamma
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Cholera Toxin
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heat-labile enterotoxin, E coli