A physical and a computational fluid dynamic (CFD) model (HYDRO-3D) were developed to simulate the effects of novel maturation pond configurations, and critical environmental factors (wind speed and direction) on the hydraulic efficiency (HE) of full-scale maturation ponds. The aims of the study were to assess the reliability of the physical model and convergence with HYDRO-3D, as tools for assessing and predicting best hydraulic performance of ponds. The physical model of the open ponds was scaled to provide a similar nominal retention time (NRT) of 52 hours. Under natural conditions, with a variable prevailing westerly wind opposite to the inlet, a rhodamine tracer study on the full-scale prototype pond produced a mean hydraulic retention time (MHRT) of 18.5 hours (HE = 35.5%). Simulations of these wind conditions, but with constant wind speed and direction in both the physical model and HYDRO-3D, produced a higher MHRT of 21 hours in both models and an HE of 40.4%. In the absence of wind tracer studies in the open pond physical model revealed incomplete mixing with peak concentrations leaving the model in several hours, but an increase in MHRT to 24.5-28 hours (HE = 50.2-57.1%). Although wind blowing opposite to the inlet flow increases dispersion (mixing), it reduced hydraulic performance by 18-25%. Much higher HE values were achieved by baffles (67-74%) and three channel configurations (69-92%), compared with the original open pond configuration. Good agreement was achieved between the two models where key environmental and flow parameters can be controlled and set, but it is difficult to accurately simulate full-scale works conditions due to the unpredictability of natural hourly and daily fluctuation in these parameters.