Objective: To investigate the effect of bone marrow fibroblasts conditioned media on the expansion of megakaryocytes in vitro.
Methods: Serum-free bone marrow fibroblasts conditioned medium (BMF-CM) was prepared and collected. The effect of BMF-CM or BMF-CM combined with IL-11 on the expansion of mature megakaryocytes and the effect of BMF-CM on the expansion of megakaryocytic progenitors were observed. RT-PCR was applied to determine the expression of NF-E2 mRNA in human megakaryoblastic leukemia cell line--Meg-0l.
Results: BMF-CM promoted the expansion of mature megakaryocytes. The effect of BMF-CM combined with IL-11 on the expansion of mature megakaryocytes was further enhanced. BMF-CM promoted the expansion of megakaryocytic progenitors. NF-E2 mRNA expression in megakaryocytic cell lines Meg-0l was enhanced when cultures were exposed to 30% BMF-CM for 4 hours, compared with control cultures exposed to IMDM for 4 hours.
Conclusion: BMF-CM can promote the expansion of megakaryocytes in vitro.