Objective: To observe the differentially expressed genes of the human embryo lung fibroblast (MRC-5) induced by formaldehyde (FA) of low dose using fluoro DD-PCR.
Methods: The dose-effect relation of FA toxicity to MRC-5 was acquired, No observed damage effect or proliferation concentration was used as low dose, and obvious damage concentration was used as high dose. MRC-5 was treated with low and high dose, and treated with high dose after pretreated with low dose for some time. Then Fluoro differential display polymerase chain reaction (Fluoro DD-PCR) was used to search differentially expressed genes of the differently treated groups of FA. 61 differential display straps were acquired and 11 of them were reamplified, cloned, sequenced and blasted.
Results: According to the dose-effect relation of FA toxicity to MRC-5, 100 micromol/L was choosed as low dose and 10 mmol/L was choosed as high dose. Samples of differently treated groups were amplified by means of fluro-DD-PCR, 61 differentially expressed straps were acquired. 11 differential display straps have been cloned, sequenced and blasted. Two of them were known genes: one was highly homologous to nuclear factor of activated T-cells 5 (NFAT5) and the other was highly homologous to tetratricopeptide repeat domain 3(TPRD-3). Nine of them were new genes.
Conclusion: It seemed that FA of low dose could promote MRC-5 proliferation and find 61 differentially expressed genes of differently treated groups, and the result of clone could provide scientific base for mechanism of FA toxicity research.