Abstract
In this study, micellar electrokinetic chromatography (MEKC) method was developed for the determination of clotrimazole (CLO), methylparaben (MP) and propylparaben (PP) in a pharmaceutical preparation. Separation was carried out in a fused silica capillary (60 cm x 75 microm i.d.) at 25 kV with UV detection at 212 nm. Optimized background electrolyte (BGE) was 15 mM phosphate buffer (pH 7.2) containing 30 mM sodium dodecyl sulfate (SDS) as a surfactant. Rectilinear calibration ranges were 50-500 mg l(-1) for CLO, 10-100 mg l(-1) for MP and 2.5-25 mg l(-1) for PP. The total analysis time was < 12 min.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Infective Agents, Local / analysis
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Anti-Infective Agents, Local / chemistry
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Calibration
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Chemistry Techniques, Analytical / methods*
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Chemistry, Pharmaceutical / methods*
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Chromatography, High Pressure Liquid
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Chromatography, Micellar Electrokinetic Capillary / methods*
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Clotrimazole / analysis*
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Clotrimazole / chemistry
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Electrolytes
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Hydrogen-Ion Concentration
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Models, Chemical
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Parabens / analysis*
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Parabens / chemistry
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Phosphates / analysis
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Preservatives, Pharmaceutical / analysis
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Reproducibility of Results
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Sensitivity and Specificity
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Sodium Dodecyl Sulfate / chemistry
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Surface-Active Agents / analysis
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Temperature
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Time Factors
Substances
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Anti-Infective Agents, Local
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Electrolytes
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Parabens
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Phosphates
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Preservatives, Pharmaceutical
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Surface-Active Agents
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Sodium Dodecyl Sulfate
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methylparaben
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Clotrimazole
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propylparaben