Excited-state structure determination of the green fluorescent protein chromophore

Anwar Usman; Omar F Mohammed; Erik T J Nibbering; Jian Dong; Kyril M Solntsev; Laren M Tolbert

doi:10.1021/ja0520520

Excited-state structure determination of the green fluorescent protein chromophore

J Am Chem Soc. 2005 Aug 17;127(32):11214-5. doi: 10.1021/ja0520520.

Authors

Anwar Usman¹, Omar F Mohammed, Erik T J Nibbering, Jian Dong, Kyril M Solntsev, Laren M Tolbert

Affiliation

¹ Max Born Institut für Nichtlineare Optik und Kurzzeitspektroskopie, Max Born Strasse 2A, D-12489 Berlin, Germany.

PMID: 16089429
DOI: 10.1021/ja0520520

Abstract

Ultrafast polarization-sensitive infrared (IR) spectroscopy of the C=O stretching mode of the chromophore of the green fluorescent protein reveals a near complete twisting around the ethylenic bridge between the phenolate and imidazolidinone groups upon electronic excitation, hinting at a decisive role of this motion in the efficient internal conversion process.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Green Fluorescent Proteins / chemistry*
Luminescent Proteins / chemistry*
Protein Conformation
Spectrophotometry, Infrared

Substances

Luminescent Proteins
Green Fluorescent Proteins