Induced accumulation of oleanolic acid and ursolic acid in cell suspension cultures of Uncaria tomentosa

Biotechnol Lett. 2005 Jun;27(12):839-43. doi: 10.1007/s10529-005-6215-7.

Abstract

Increasing sucrose from 20 to 50 g l(-1) in Uncaria tomentosa cell suspension cultures enhanced ursolic acid and oleanolic acid production from 129 +/- 61 to 553 +/- 193 microg g(-1) cell dry wt. The maximal concentration of both triterpenes (1680 +/- 39 microg g(-1) cell dry wt) was 8 days after elicitation by jasmonic acid, while yeast extract or citrus pectin treatments produced 1189 +/- 20 or 1120 +/- 26 microg g(-1) cell dry wt, respectively. The ratio of ursolic acid:oleanolic acid was constant at 70:30.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomass
  • Cat's Claw / drug effects
  • Cat's Claw / growth & development
  • Cat's Claw / metabolism*
  • Cell Culture Techniques / methods
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Molecular Structure
  • Oleanolic Acid / chemistry
  • Oleanolic Acid / metabolism*
  • Sucrose / pharmacology
  • Time Factors
  • Triterpenes / chemistry
  • Triterpenes / metabolism*
  • Ursolic Acid

Substances

  • Triterpenes
  • Sucrose
  • Oleanolic Acid