Abstract
Insertional inactivation of the plasmid-encoded determinants for Mg(2+) and Co(2+) transport, orf18/corA, provides a tool for screening recombinant clones in Lactococcus, based on the observation that overexpression of orf18/corA results in cell growth inhibition on certain concentrations of CoCl(2). The lacticin 3147 immunity gene, ltnI, was used to insertionally inactivate orf18/corA. The resulting clones were capable of growth on concentrations of CoCl(2) that were inhibitory to the parent strain. Since only 3 of 17 lactococcal starters naturally harbor corA, the system has potential as a screen for selecting recombinant lactococcal clones.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Biological Transport, Active
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Cation Transport Proteins / genetics*
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Cation Transport Proteins / metabolism
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Cloning, Molecular
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Cobalt / metabolism*
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Cobalt / pharmacology
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Gene Expression Regulation, Bacterial
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Lactococcus / classification
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Lactococcus / genetics
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Lactococcus / growth & development
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Lactococcus / metabolism*
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Magnesium / metabolism*
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Magnesium / pharmacology
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Mutagenesis, Insertional*
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Open Reading Frames
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Plasmids
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Recombinant Proteins / genetics*
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Recombinant Proteins / metabolism
Substances
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Bacterial Proteins
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Cation Transport Proteins
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CorA protein, Salmonella
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Recombinant Proteins
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Cobalt
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Magnesium