Primers for clinical detection of Paracoccidioides brasiliensis

J Clin Microbiol. 2005 Aug;43(8):4255-7. doi: 10.1128/JCM.43.8.4255-4257.2005.

Abstract

From a 0.72-kb fragment universally generated in Paracoccidioides brasiliensis strains, primers were designed and tested on genomic DNA of this and other pathogenic fungi. They were specific and highly sensitive for P. brasiliensis DNA. Positive results were obtained when these were tested in clinical samples.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • DNA Primers*
  • DNA, Fungal / analysis*
  • Female
  • Humans
  • Male
  • Middle Aged
  • Paracoccidioides / genetics
  • Paracoccidioides / isolation & purification*
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity

Substances

  • DNA Primers
  • DNA, Fungal