Comparison between sequence analysis and a line probe assay for testing genotypic resistance of human immunodeficiency virus type 1 to antiretroviral drugs

S García-Bujalance; C Ladrón de Guevara; J González-García; J R Arribas; A Gutiérrez

doi:10.1128/JCM.43.8.4186-4188.2005

Comparison between sequence analysis and a line probe assay for testing genotypic resistance of human immunodeficiency virus type 1 to antiretroviral drugs

J Clin Microbiol. 2005 Aug;43(8):4186-8. doi: 10.1128/JCM.43.8.4186-4188.2005.

Authors

S García-Bujalance¹, C Ladrón de Guevara, J González-García, J R Arribas, A Gutiérrez

Affiliation

¹ Department of Microbiology and Parasitology, La Paz University Hospital, Paseo de la Castellana 261, 28046 Madrid, Spain. sgarciab.hulp@salud.madrid.org

Abstract

The purpose of this study was to compare a line probe assay (LiPA) with sequence analysis for the detection of mutations conferring resistance to nucleoside and non-nucleoside inhibitors in human immunodeficiency reverse transcriptase and protease inhibitors. The limitations for interpreting LiPA make it unacceptable for routine clinical practice.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Anti-HIV Agents / pharmacology*
Base Sequence
Drug Resistance, Viral
Genotype
HIV-1 / classification
HIV-1 / drug effects*
HIV-1 / genetics
Humans
Molecular Sequence Data
Sequence Analysis, DNA

Substances

Anti-HIV Agents

Associated data

GENBANK/AY872282
GENBANK/AY872335
GENBANK/AY872336
GENBANK/AY872389