Spylidone, a novel inhibitor of lipid droplet accumulation in mouse macrophages produced by Phoma sp. FKI-1840

J Antibiot (Tokyo). 2005 May;58(5):338-45. doi: 10.1038/ja.2005.42.

Abstract

During our screening for microbial inhibitors of lipid droplet accumulation in macrophages, a new compound designated spylidone was isolated along with two structurally related known compounds, PF1052 and vermisporin, from the fermentation broth of Phoma sp. FKI-1840 by solvent extraction, silica gel column chromatography, ODS column chromatography and preparative HPLC. From the structure elucidation, spylidone has a spiro ring containing 2,4-pyrrolidinedione. Among the three compounds, only spylidone was found to inhibit lipid droplet accumulation in macrophages at 10 to approximately 50 microM without any cytotoxic effect.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Ascomycota / chemistry*
  • Ascomycota / classification
  • Bacteria / drug effects
  • Cell Line
  • Chemical Phenomena
  • Chemistry, Physical
  • Fermentation
  • Fungi / drug effects
  • Lipid Metabolism*
  • Macrophages / drug effects
  • Macrophages / metabolism*
  • Mice
  • Microbial Sensitivity Tests
  • Molecular Conformation
  • Phenanthrenes / pharmacology*
  • Soil Microbiology
  • Spectrometry, Mass, Fast Atom Bombardment
  • Spectrophotometry, Infrared
  • Spectrophotometry, Ultraviolet
  • Spiro Compounds / pharmacology*

Substances

  • Phenanthrenes
  • Spiro Compounds
  • spylidone