Inducement and reversal of tetracycline resistance in Escherichia coli K-12 and expression of proton gradient-dependent multidrug efflux pump genes

Antimicrob Agents Chemother. 2005 Aug;49(8):3578-82. doi: 10.1128/AAC.49.8.3578-3582.2005.

Abstract

Expression of eight transporter genes of Escherichia coli K-12 and its DeltaacrAB mutant prior to and after induction of both strains to tetracycline resistance and after reversal of induced resistance were analyzed by quantitative reverse transcriptase PCR. All transporter genes were overexpressed after induced resistance with acrF being 80-fold more expressed in the DeltaacrAB tetracycline-induced strain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism
  • Drug Resistance, Multiple, Bacterial / genetics*
  • Escherichia coli K12 / drug effects*
  • Escherichia coli Proteins / genetics
  • Escherichia coli Proteins / metabolism*
  • Gene Expression Regulation, Bacterial*
  • Microbial Sensitivity Tests
  • Mutation
  • Proton Pumps / metabolism*
  • Tetracycline / pharmacology
  • Tetracycline Resistance*

Substances

  • Anti-Bacterial Agents
  • Carrier Proteins
  • Escherichia coli Proteins
  • Proton Pumps
  • Tetracycline