Allosterically activated Diels-Alder catalysis by a ribozyme

J Am Chem Soc. 2005 Aug 3;127(30):10492-3. doi: 10.1021/ja052886i.

Abstract

We describe the allosteric control of Diels-Alder reactions by a small organic effector, theophylline. This is achieved by converting a Diels-Alder ribozyme into an allosterically regulated system. In contrast to other published systems, we have a bond-forming reaction with two small-molecule substrates and multiple turnover. This system could be very attractive for the development of assays for a variety of analytes and can be regarded as a prototype of fully synthetic signaling cascades.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anthracenes / chemistry*
  • Base Sequence
  • Biotin / chemistry
  • Catalysis
  • DNA-Directed RNA Polymerases / chemistry
  • Kinetics
  • Maleimides / chemistry*
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Nucleotides / chemistry
  • RNA / chemistry*
  • RNA, Catalytic / chemistry*
  • RNA, Catalytic / metabolism
  • Theophylline / chemistry
  • Viral Proteins / chemistry

Substances

  • Anthracenes
  • Maleimides
  • Nucleotides
  • RNA, Catalytic
  • Viral Proteins
  • RNA
  • Biotin
  • Theophylline
  • bacteriophage T7 RNA polymerase
  • DNA-Directed RNA Polymerases
  • anthracene