NF-kappa B subunit regulation in nontransformed CD4+ T lymphocytes

Science. 1992 Jun 5;256(5062):1452-6. doi: 10.1126/science.1604322.

Abstract

Regulation of interleukin-2 (IL-2) gene expression by the p50 and p65 subunits of the DNA binding protein NF-kappa B was studied in nontransformed CD4+ T lymphocyte clones. A homodimeric complex of the NF-kappa B p50 subunit was found in resting T cells. The amount of p50-p50 complex decreased after full antigenic stimulation, whereas the amount of the NF-kappa B p50-p65 heterodimer was increased. Increased expression of the IL-2 gene and activity of the IL-2 kappa B DNA binding site correlated with a decrease in the p50-p50 complex. Overexpression of p50 repressed IL-2 promoter expression. The switch from p50-p50 to p50-p65 complexes depended on a protein that caused sequestration of the p50-p50 complex in the nucleus.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Binding Sites
  • CD4 Antigens / immunology*
  • Cell Line
  • Cell Nucleus / physiology
  • Chloramphenicol O-Acetyltransferase / genetics
  • Chloramphenicol O-Acetyltransferase / metabolism
  • Clone Cells
  • Columbidae
  • DNA / genetics
  • Gene Expression Regulation*
  • Interleukin-2 / genetics*
  • Macromolecular Substances
  • Mice
  • Molecular Sequence Data
  • NF-kappa B / metabolism*
  • Oligonucleotide Probes
  • Promoter Regions, Genetic
  • RNA, Messenger / metabolism
  • Recombinant Fusion Proteins / metabolism
  • T-Lymphocyte Subsets / immunology*
  • Transfection
  • Tumor Necrosis Factor-alpha / pharmacology

Substances

  • CD4 Antigens
  • Interleukin-2
  • Macromolecular Substances
  • NF-kappa B
  • Oligonucleotide Probes
  • RNA, Messenger
  • Recombinant Fusion Proteins
  • Tumor Necrosis Factor-alpha
  • DNA
  • Chloramphenicol O-Acetyltransferase