Abstract
Aldosterone is a mineralocorticoid hormone that regulates blood pressure and salt/water balance. Increased aldosterone levels are found in states of disturbed energy balance such as the metabolic syndrome. Adipose tissue has been recognized to play a pivotal role in the regulation of energy homeostasis. We investigated direct aldosterone effects on brown adipocyte function. Aldosterone dose-dependently inhibited expression of uncoupling protein-1 (UCP-1) by 30% (p < 0.01). Furthermore, aldosterone dose-dependently impaired insulin-induced glucose uptake by about 25% (p < 0.01). On a transcriptional level, mRNA of the proinflammatory adipokines leptin and monocyte chemoattractant protein-1 (MCP-1) was increased by 5,000% and 40%, respectively, by aldosterone exposure (p < 0.05). This study demonstrates that aldosterone directly impacts on major adipose functions including stimulation of proinflammatory adipokines.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adipocytes / drug effects
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Adipocytes / metabolism*
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Adipose Tissue, Brown / cytology
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Adipose Tissue, Brown / drug effects
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Adipose Tissue, Brown / metabolism
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Aldosterone / pharmacology*
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Animals
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Blotting, Western
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Carrier Proteins / antagonists & inhibitors*
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Chemokine CCL2 / biosynthesis
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Dose-Response Relationship, Drug
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Energy Metabolism / drug effects
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Gene Expression / drug effects
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Glucose / metabolism
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Inflammation / pathology*
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Insect Hormones / biosynthesis*
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Insulin Resistance / physiology*
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Ion Channels
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Leptin / biosynthesis
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Membrane Proteins / antagonists & inhibitors*
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Mice
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Mitochondrial Proteins
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Oligopeptides / biosynthesis*
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Pyrrolidonecarboxylic Acid / analogs & derivatives*
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RNA, Messenger / biosynthesis
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Thermogenesis / drug effects
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Uncoupling Protein 1
Substances
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Carrier Proteins
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Chemokine CCL2
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Insect Hormones
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Ion Channels
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Leptin
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Membrane Proteins
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Mitochondrial Proteins
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Oligopeptides
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RNA, Messenger
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Ucp1 protein, mouse
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Uncoupling Protein 1
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adipokinetic hormone
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Aldosterone
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Glucose
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Pyrrolidonecarboxylic Acid