The purpose of the present study was to evaluate in vitro, the influence of two different methods of surfaces conditioning of dentin by tetracycline hydrochloride on human gingival epithelial cell attachment. A flat dentin surface was created on human teeth extracted due to severe periodontitis: 36 specimens were divided into 3 equal groups and etched in the following way: group 1 (saline); group 2 (immersion in TTC for 3 min); group 3 (burnishing with TTC for 3 min). The specimens and fibroblasts were incubated in a culture medium for 24 hours, one and two weeks and photographed using scanning electron microscope. No fibroblasts could be seen on the saline groups. More fibroblasts could attach to the root surface at one and two weeks irrespective of the method used.