Abstract
Objective:
To explore the role of BM2 protein in the life cycle of influenza B virus.
Methods:
The authors screened human kidney MATCHMAKER cDNA library for new binding partners of BM2 of influenza B virus by using the yeast two hybrid system with truncated BM2 (26-109 aa) as the bait.
Results:
Six positive plasmids encoding N-acetylneuraminate pyruvate lyase, angiopoietin 3, zinc finger protein 251, ribosomal protein S20, protein arginine N-methyltransferase 1 variant 1 (PRMT) and transcription factor-like 1 (TCFL1) were obtained.
Conclusion:
The results suggest that BM2 may play an important role in the life cycle of influenza B virus.
MeSH terms
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Angiopoietin-Like Protein 1
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Angiopoietin-like Proteins
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Angiopoietins / genetics
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Angiopoietins / metabolism
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Gene Library
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Humans
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Influenza B virus / genetics
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Influenza B virus / metabolism*
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Kidney / metabolism
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Oxo-Acid-Lyases / genetics
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Oxo-Acid-Lyases / metabolism
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Plasmids / genetics
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Protein Binding
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Protein-Arginine N-Methyltransferases / genetics
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Protein-Arginine N-Methyltransferases / metabolism
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Repressor Proteins / genetics
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Repressor Proteins / metabolism
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Ribosomal Proteins / genetics
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Ribosomal Proteins / metabolism
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Transcription Factors / genetics
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Transcription Factors / metabolism
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Two-Hybrid System Techniques*
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Viral Proteins / genetics
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Viral Proteins / metabolism*
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Zinc Fingers / genetics
Substances
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ANGPTL1 protein, human
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Angiopoietin-Like Protein 1
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Angiopoietin-like Proteins
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Angiopoietins
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BM2 polypeptide, influenza B virus
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DNA-Binding Proteins
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Repressor Proteins
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Ribosomal Proteins
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Transcription Factors
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VPS72 protein, human
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Viral Proteins
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ribosomal protein S20
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PRMT1 protein, human
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Protein-Arginine N-Methyltransferases
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Oxo-Acid-Lyases
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N-acetylneuraminate lyase