Abstract
PedB, a bacterial immunity protein conferring immunity to a newly identified pediocin (pediocin PP-1), was crystallized by the hanging-drop vapor diffusion method at 296 K. A 1.35 A data set has been collected from a single crystal at 100 K using synchrotron-radiation source. The PedB crystals belong to the hexagonal space group P6(2) or P6(4), with unit cell parameters a = b = 62.2, c = 39.9 A. Analysis of the packing density shows that the asymmetric unit probably contains one molecule with a solvent content of 33.8%.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / biosynthesis
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Bacterial Proteins / chemistry*
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Bacterial Proteins / genetics
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Crystallization
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Crystallography, X-Ray
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Escherichia coli / genetics
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Genetic Vectors / genetics
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Molecular Sequence Data
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Pediococcus / chemistry
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Pediococcus / genetics*
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry*
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Sequence Homology, Amino Acid
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Transformation, Genetic
Substances
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Bacterial Proteins
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PedB protein, Pediococcus pentosaceus
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Recombinant Proteins