Abstract
Fourteen strains isolated from vegetable sources and identified as belonging to Lactobacillus plantarum presented an atypical pattern of amplification with a species-specific multiplex-PCR assay. Phylogenetic analysis of two protein-encoding genes, recA (encoding the recombinase A protein) and cpn60 (encoding the GroEL chaperonin), as well as phenotypic and genomic traits revealed a homogeneous group of very closely related strains for which subspecies status is proposed, with the name Lactobacillus plantarum subsp. argentoratensis. The type strain is DKO 22(T) (=CIP 108320(T)=DSM 16365(T)).
MeSH terms
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Bacterial Typing Techniques
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Chaperonin 60 / genetics
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DNA, Bacterial / analysis
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DNA, Ribosomal / analysis
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Genes, rRNA
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Lactobacillus plantarum / classification*
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Lactobacillus plantarum / genetics
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Lactobacillus plantarum / isolation & purification*
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Lactobacillus plantarum / physiology
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Molecular Sequence Data
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Phenotype
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Phylogeny
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Polymerase Chain Reaction
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RNA, Ribosomal, 16S / genetics
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Rec A Recombinases / genetics
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Sequence Analysis, DNA
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Species Specificity
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Vegetables / microbiology*
Substances
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Chaperonin 60
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DNA, Bacterial
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DNA, Ribosomal
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RNA, Ribosomal, 16S
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Rec A Recombinases
Associated data
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GENBANK/AJ621662
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GENBANK/AJ621666
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GENBANK/AJ621668
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GENBANK/AJ640078
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GENBANK/AJ640079
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GENBANK/AJ640080
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GENBANK/AJ640081
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GENBANK/AJ640082