A sensitive and specific high performance liquid chromatographic assay for imidazole dipeptides and 3-methylhistidine in human muscle biopsies, serum and urine

Biomed Chromatogr. 1992 Jan-Feb;6(1):16-9. doi: 10.1002/bmc.1130060106.

Abstract

A sensitive and specific high performance liquid chromatographic method is described for measuring imidazole dipeptides and 3-methylhistidine in human muscle biopsies, serum and urine. Muscle extract, serum or urine was reacted with o-phthaldialdehyde and the derivatives were separated by reversed phase chromatography with column switching and fluorescence detection.

MeSH terms

  • Anserine / blood
  • Anserine / metabolism*
  • Anserine / urine
  • Biopsy
  • Carnosine / blood
  • Carnosine / metabolism*
  • Carnosine / urine
  • Chromatography, High Pressure Liquid
  • Humans
  • Methylhistidines / blood
  • Methylhistidines / metabolism*
  • Methylhistidines / urine
  • Muscles / chemistry*
  • Muscles / pathology
  • Spectrometry, Fluorescence

Substances

  • Methylhistidines
  • Carnosine
  • Anserine
  • 3-methylhistidine