Abstract
A sensitive and specific high performance liquid chromatographic method is described for measuring imidazole dipeptides and 3-methylhistidine in human muscle biopsies, serum and urine. Muscle extract, serum or urine was reacted with o-phthaldialdehyde and the derivatives were separated by reversed phase chromatography with column switching and fluorescence detection.
MeSH terms
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Anserine / blood
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Anserine / metabolism*
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Anserine / urine
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Biopsy
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Carnosine / blood
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Carnosine / metabolism*
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Carnosine / urine
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Chromatography, High Pressure Liquid
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Humans
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Methylhistidines / blood
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Methylhistidines / metabolism*
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Methylhistidines / urine
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Muscles / chemistry*
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Muscles / pathology
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Spectrometry, Fluorescence
Substances
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Methylhistidines
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Carnosine
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Anserine
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3-methylhistidine