Abstract
The resolution of asparagine-type oligosaccharides in glycoproteins was carried out by combination of the transglycosylation reaction and CE-TOF-MS. The oligosaccharides enzymatically transferred to a fluorescent acceptor (NDA-Asn-GlcNAc) with Endo-M. The resulting fluorescent-oligosaccharides were separated by CE and detected by TOF-MS. Disialo-Asn was successfully identified by the proposed procedure. Application to oligosaccharides in ovalbumin was also described in this communication.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylglucosamine / analogs & derivatives*
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Acetylglucosamine / chemistry
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Asparagine / analogs & derivatives*
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Asparagine / chemistry
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Electrophoresis, Capillary / methods*
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Fluorescence
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Glycopeptides / chemistry
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Glycoproteins / chemistry*
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Glycosylation
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Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase / chemistry
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Oligosaccharides / analysis*
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Substances
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Glycopeptides
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Glycoproteins
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Oligosaccharides
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Asparagine
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Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
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Acetylglucosamine