Enhancement of glutamine utilization in Bacillus subtilis through the GlnK-GlnL two-component regulatory system

J Bacteriol. 2005 Jul;187(14):4813-21. doi: 10.1128/JB.187.14.4813-4821.2005.

Abstract

During DNA microarray analysis, we discovered that the GlnK-GlnL (formerly YcbA-YcbB) two-component system positively regulates the expression of the glsA-glnT (formerly ybgJ-ybgH) operon in response to glutamine in the culture medium on Northern analysis. As a result of gel retardation and DNase I footprinting analyses, we found that the GlnL protein interacts with a region (bases -13 to -56; +1 is the transcription initiation base determined on primer extension analysis of glsA-glnT) in which a direct repeat, TTTTGTN4TTTTGT, is present. Furthermore, the glsA and glnT genes were biochemically verified to encode glutaminase and glutamine transporter, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus subtilis / genetics
  • Bacillus subtilis / metabolism*
  • Bacterial Proteins / metabolism
  • Base Sequence
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • DNA Primers
  • Genes, Reporter
  • Genotype
  • Glutaminase / genetics
  • Glutaminase / metabolism*
  • Glutamine / metabolism*
  • Histidine Kinase
  • Kinetics
  • Molecular Sequence Data
  • Protein Kinases / genetics
  • Protein Kinases / metabolism*
  • Trans-Activators / genetics
  • Trans-Activators / metabolism*
  • beta-Galactosidase / genetics
  • beta-Galactosidase / metabolism

Substances

  • Bacterial Proteins
  • Carrier Proteins
  • DNA Primers
  • Trans-Activators
  • glutamine transport proteins
  • Glutamine
  • Protein Kinases
  • Histidine Kinase
  • beta-Galactosidase
  • Glutaminase