Abstract
During DNA microarray analysis, we discovered that the GlnK-GlnL (formerly YcbA-YcbB) two-component system positively regulates the expression of the glsA-glnT (formerly ybgJ-ybgH) operon in response to glutamine in the culture medium on Northern analysis. As a result of gel retardation and DNase I footprinting analyses, we found that the GlnL protein interacts with a region (bases -13 to -56; +1 is the transcription initiation base determined on primer extension analysis of glsA-glnT) in which a direct repeat, TTTTGTN4TTTTGT, is present. Furthermore, the glsA and glnT genes were biochemically verified to encode glutaminase and glutamine transporter, respectively.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacillus subtilis / genetics
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Bacillus subtilis / metabolism*
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Bacterial Proteins / metabolism
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Base Sequence
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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DNA Primers
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Genes, Reporter
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Genotype
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Glutaminase / genetics
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Glutaminase / metabolism*
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Glutamine / metabolism*
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Histidine Kinase
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Kinetics
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Molecular Sequence Data
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Protein Kinases / genetics
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Protein Kinases / metabolism*
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Trans-Activators / genetics
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Trans-Activators / metabolism*
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beta-Galactosidase / genetics
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beta-Galactosidase / metabolism
Substances
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Bacterial Proteins
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Carrier Proteins
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DNA Primers
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Trans-Activators
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glutamine transport proteins
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Glutamine
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Protein Kinases
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Histidine Kinase
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beta-Galactosidase
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Glutaminase