Simple species identification of Trichinella isolates by amplification and sequencing of the 5S ribosomal DNA intergenic spacer region

Aymeric De Bruyne; Hélène Yera; Franck Le Guerhier; Pascal Boireau; Jean Dupouy-Camet

doi:10.1016/j.vetpar.2005.05.026

Simple species identification of Trichinella isolates by amplification and sequencing of the 5S ribosomal DNA intergenic spacer region

Vet Parasitol. 2005 Sep 5;132(1-2):57-61. doi: 10.1016/j.vetpar.2005.05.026.

Authors

Aymeric De Bruyne¹, Hélène Yera, Franck Le Guerhier, Pascal Boireau, Jean Dupouy-Camet

Affiliation

¹ Trichinella National Reference Center, Assistance Publique-Hôpitaux de Paris, Hôpital Cochin, R. Descartes University, 27 rue du Fbrg St. Jacques, 75014 Paris, France.

PMID: 15992998
DOI: 10.1016/j.vetpar.2005.05.026

Abstract

We developed a PCR-based assay using a single primer pair to amplify the 5S ribosomal DNA intergenic spacer region to identify Trichinella isolates. In our method, amplified products are directly sequenced on both strands and compared to GenBank sequences. Using this method, we were able to identify Trichinella spiralis, T. britovi and T. nativa. This method permits rapid species identification of Trichinella isolates; however, further evaluation is required before recommending this approach for routine use.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
DNA, Helminth / chemistry
DNA, Helminth / genetics
DNA, Ribosomal Spacer / chemistry
DNA, Ribosomal Spacer / genetics
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
RNA, Ribosomal, 5S / chemistry
RNA, Ribosomal, 5S / genetics*
Sequence Alignment
Trichinella / classification*
Trichinella / genetics

Substances

DNA, Helminth
DNA, Ribosomal Spacer
RNA, Ribosomal, 5S