Correction of X-ray intensities from an HslV-HslU co-crystal containing lattice-translocation defects

Acta Crystallogr D Biol Crystallogr. 2005 Jul;61(Pt 7):932-41. doi: 10.1107/S0907444905009546. Epub 2005 Jun 24.

Abstract

Because of lattice-translocation defects, two identical but translated lattices can coexist as a single coherent mosaic block in a crystal. The observed structure in such cases is a weighted sum of two identical but translated structures, one from each lattice; the observed structure factors are a weighted vector sum of the structure factors with identical unit amplitudes but shifted phases. The correction of X-ray intensities from a single crystal containing these defects of the hybrid HslV-HslU complex, which consists of Escherichia coli HslU and Bacillus subtilis HslV (also known as CodW), is reported. When intensities are not corrected, a biologically irrelevant complex (with CodW from one lattice and HslU from another) is implied to exist. Only upon correction does a biologically functional CodW-HslU complex structure emerge.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Crystallization
  • Crystallography, X-Ray / methods*
  • Endopeptidase Clp / chemistry*
  • Escherichia coli Proteins / chemistry*
  • Models, Molecular
  • Protein Structure, Quaternary
  • Serine Endopeptidases / chemistry*

Substances

  • ClpYQ protease, E coli
  • Escherichia coli Proteins
  • CodWX protease
  • Serine Endopeptidases
  • Endopeptidase Clp