A public software for energy filtering transmission electron tomography (EFTET-J): application to the study of granular inclusions in bacteria from Riftia pachyptila

J Struct Biol. 2005 Aug;151(2):151-9. doi: 10.1016/j.jsb.2005.05.004.

Abstract

Energy-filtering transmission electron microscopy (EFTEM) allows the determination of elemental distributions out of a sequence of energy filtered images. Combined with electron tomography, EFTEM is a powerful tool to obtain three-dimensional chemical maps from sub-cellular structures. However, there is no existing software in the public-domain for the computation and analysis of 3D-chemical maps. Here, we present a Java-based program to compute 3D-elemental distribution. This program is available as a set of plug-ins for the public-domain Java image processing program Image J inspired by NIH Image. Its implemented algorithms have been successfully applied to the three-dimensional localization of iron granules in semi thin (200 nm) epon sections from the vent worm Riftia pachyptalia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Animals
  • Bacteria / cytology*
  • Frozen Sections
  • Image Processing, Computer-Assisted
  • Imaging, Three-Dimensional
  • Inclusion Bodies / chemistry*
  • Iron / analysis
  • Microscopy, Energy-Filtering Transmission Electron*
  • Polychaeta / microbiology*
  • Software*
  • Tomography*

Substances

  • Iron