Abstract
Stem cell factor is an important hematopoietic growth factor. In this study, the human stem cell factor was produced by recombinant E. coli, and the structure and biological activity of the recombinant stem cell factor(rhSCF) was studied. It was indicated that the rhSCF was a uncovalent dimer in phosphate buffer,and had the correct mass spectra, mass peptides spectra, composition of amino acid, N-terminal sequernce, C-terminal sequence and intrachain disulfide linkages, rhSCF alone or synergy with rhG-CSF could mobilze hematopoietic progenitors to blood in monkey.
Publication types
-
English Abstract
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Cell Differentiation / drug effects
-
Cells, Cultured
-
Chromatography, High Pressure Liquid
-
Haplorhini
-
Hematopoietic Stem Cells / cytology
-
Hematopoietic Stem Cells / drug effects
-
Humans
-
Recombinant Proteins / chemistry
-
Recombinant Proteins / genetics
-
Recombinant Proteins / metabolism*
-
Recombinant Proteins / pharmacology*
-
Sequence Analysis, Protein
-
Spectrometry, Mass, Fast Atom Bombardment
-
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
-
Stem Cell Factor / chemistry
-
Stem Cell Factor / genetics
-
Stem Cell Factor / metabolism*
-
Stem Cell Factor / pharmacology*
Substances
-
Recombinant Proteins
-
Stem Cell Factor