Abstract
We isolated and characterized the Xenopus translocon-associated protein XTRAP-gamma, one of four subunits of the translocon-associated protein complex. TRAP has been proposed to aid the translocation of nascent polypeptides into the lumen of the endoplasmic reticulum, but this has not been demonstrated until now. XTRAP-gamma was specifically expressed in the pronephros tubules of Xenopus laevis from stage 25 during kidney development. Antisense morpholino oligonucleotide-mediated knockdown of XTRAP-gamma suppressed tubulogenesis and decreased expression of the pronephros marker genes Pax-2 and Wnt-4. XTRAP-gamma morpholinos also inhibited differentiation of the pronephros in activin/retinoic acid-treated animal caps. We conclude that XTRAP-gamma plays an important role in the process of pronephros differentiation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Activins / metabolism
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Amino Acid Sequence
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Animals
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Calcium-Binding Proteins / chemistry
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Calcium-Binding Proteins / genetics
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Calcium-Binding Proteins / physiology*
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Embryo, Nonmammalian / physiology*
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Gene Expression Regulation, Developmental
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Gene Library
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Homeodomain Proteins
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In Situ Hybridization
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Inhibins
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Kidney / embryology*
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Membrane Glycoproteins / chemistry
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / physiology*
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Molecular Sequence Data
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Oligonucleotides, Antisense
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Receptors, Cytoplasmic and Nuclear / chemistry
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Receptors, Cytoplasmic and Nuclear / genetics
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Receptors, Cytoplasmic and Nuclear / physiology*
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Receptors, Peptide / chemistry
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Receptors, Peptide / genetics
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Receptors, Peptide / physiology*
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Up-Regulation
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Xenopus Proteins / genetics
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Xenopus Proteins / physiology*
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Xenopus laevis / embryology*
Substances
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Calcium-Binding Proteins
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Homeodomain Proteins
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Membrane Glycoproteins
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Oligonucleotides, Antisense
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Receptors, Cytoplasmic and Nuclear
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Receptors, Peptide
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Xenopus Proteins
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signal sequence receptor
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Activins
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Inhibins