Production of glucuronan oligosaccharides using a new glucuronan lyase activity from a Trichoderma sp. strain

J Biotechnol. 2005 Sep 10;118(4):448-57. doi: 10.1016/j.jbiotec.2005.04.014.

Abstract

Sinorhizobium meliloti M5N1CS synthesizes a homopolymer of glucuronic acids beta-(1,4) linked and variably C2 and/or C3O-acetylated. To obtain beta-Delta-(4,5)-unsaturated oligoglucuronans, various acetylated forms of this bacterial polymer were cleaved by a Trichoderma sp. GL2 glucuronan lyase. Oligomers with polymerization degrees up to 8 were then produced, purified by liquid chromatography (size exclusion and anions exchange) and characterized using 1H NMR and ESI-Q/TOF-MS. Finally, the production (in gram quantity) of pure unsaturated oligoglucuronans non-acetylated (di- and trisaccharide) was investigated thanks to the complete depolymerization of deacetylated glucuronan.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation
  • Chromatography, Liquid
  • Fungal Proteins / chemistry*
  • Glucuronates / chemistry
  • Glucuronates / isolation & purification*
  • Hydrolysis
  • Oligosaccharides / chemistry
  • Oligosaccharides / isolation & purification*
  • Polysaccharide-Lyases / chemistry*
  • Polysaccharides, Bacterial / chemistry
  • Polysaccharides, Bacterial / isolation & purification*
  • Sinorhizobium meliloti / chemistry*
  • Sinorhizobium meliloti / metabolism
  • Trichoderma / enzymology*

Substances

  • Fungal Proteins
  • Glucuronates
  • Oligosaccharides
  • Polysaccharides, Bacterial
  • Polysaccharide-Lyases
  • glucuronan lyase