Abstract
LFampin 265-284, derived from bovine lactoferrin, has broad-spectrum antimicrobial activity against the yeast Candida albicans and several Gram-positive and Gram-negative bacteria. A glycine substitution scan was used to identify residues that are important for its candidacidal activity. Each single substitution of a positively charged residue led to considerable reduction in candidacidal activity, for each residue to a different extent. Substitution within the helix-facilitating N-terminal sequence DLIW had less severe effect; substitution of Ile and Trp led to a somewhat reduced potency. No substantial effects were found on the propensity to adopt a helical structure or to bind to C. albicans cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Substitution*
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Animals
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Anti-Bacterial Agents / chemistry
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Anti-Bacterial Agents / pharmacology
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Antifungal Agents* / chemistry
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Antifungal Agents* / pharmacology
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Candida albicans / drug effects*
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Candida albicans / growth & development
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Cattle
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Lactoferrin / chemistry
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Lactoferrin / genetics*
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Lactoferrin / pharmacology
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Peptide Fragments / chemistry
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Peptide Fragments / genetics*
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Peptide Fragments / pharmacology
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Peptides / chemistry
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Peptides / genetics*
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Peptides / pharmacology
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Protein Structure, Secondary
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Structure-Activity Relationship
Substances
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Anti-Bacterial Agents
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Antifungal Agents
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LFampin 265-284
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Peptide Fragments
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Peptides
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Lactoferrin