Abstract
A novel method for the fluorescence detection of proteins in cells is described in the present study. Proteins are labelled by the selective biosynthetic incorporation of 5-hydroxytryptophan and the label is detected via selective two-photon excitation of the hydroxyindole and detection of its fluorescence emission at 340 nm. The method is demonstrated in this paper with images of a labelled protein in yeast cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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5-Hydroxytryptophan / chemistry
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5-Hydroxytryptophan / metabolism
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Animals
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Cells, Cultured
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Cytological Techniques / methods*
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Fluorescence
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Fluorescent Dyes / analysis*
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Microscopy, Fluorescence
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Phosphoglycerate Kinase / chemistry
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Phosphoglycerate Kinase / metabolism
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Staining and Labeling / methods*
Substances
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Fluorescent Dyes
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5-Hydroxytryptophan
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Phosphoglycerate Kinase