A novel method for observing proteins in vivo using a small fluorescent label and multiphoton imaging

Biochem J. 2005 Sep 15;390(Pt 3):787-90. doi: 10.1042/BJ20050648.

Abstract

A novel method for the fluorescence detection of proteins in cells is described in the present study. Proteins are labelled by the selective biosynthetic incorporation of 5-hydroxytryptophan and the label is detected via selective two-photon excitation of the hydroxyindole and detection of its fluorescence emission at 340 nm. The method is demonstrated in this paper with images of a labelled protein in yeast cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5-Hydroxytryptophan / chemistry
  • 5-Hydroxytryptophan / metabolism
  • Animals
  • Cells, Cultured
  • Cytological Techniques / methods*
  • Fluorescence
  • Fluorescent Dyes / analysis*
  • Microscopy, Fluorescence
  • Phosphoglycerate Kinase / chemistry
  • Phosphoglycerate Kinase / metabolism
  • Staining and Labeling / methods*

Substances

  • Fluorescent Dyes
  • 5-Hydroxytryptophan
  • Phosphoglycerate Kinase