Abstract
Mutants in the leucine zipper and basic regions of mouse c-jun were tested for transformation in chicken embryo fibroblast cultures. Reduction or elimination of the ability of Jun to dimerize or to bind to DNA severely decreased transformation. A chicken v-jun gene from which the major transactivation domain was deleted also failed to transform. We conclude that an intact leucine zipper, basic region and transactivation domain are required for Jun-induced oncogenic transformation. Coexpression of chicken c-Fos increased formation of transformed foci by Jun proteins of moderate to low oncogenic potency but had no effect on highly transforming Jun. Chicken c-Fos could also transform chicken embryo fibroblasts on its own, albeit after prolonged culture and at a low efficiency.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Blotting, Western
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Cell Transformation, Neoplastic / genetics*
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Cells, Cultured
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Chick Embryo
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Chickens
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Chloramphenicol O-Acetyltransferase / genetics
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Chloramphenicol O-Acetyltransferase / metabolism
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Genes, fos*
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Genes, jun*
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Leucine Zippers / genetics*
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Molecular Sequence Data
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Mutagenesis, Site-Directed
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Protein Biosynthesis
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Protein Conformation
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Proto-Oncogene Proteins c-fos / genetics*
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Proto-Oncogene Proteins c-jun / genetics*
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Transcription, Genetic
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Transcriptional Activation
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Transfection
Substances
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DNA-Binding Proteins
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Proto-Oncogene Proteins c-fos
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Proto-Oncogene Proteins c-jun
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Chloramphenicol O-Acetyltransferase