The article reported method of making chromosomes by callus and used in karyotype analysis of Phellodendron chinense. The results demonstrated that the chromosme amount to 80. The karyotype formula is K(2N) =80 =68m(2SAT) +6sm +6T. 34 pairs are metacentric (m), 3 pairs are submetacentric (sm) and 3 pair are terminal point (T). Two small satellites were observed on the short arms of the third pair.