Pretreatment of switchgrass by ammonia fiber explosion (AFEX)

Appl Biochem Biotechnol. 2005 Spring:121-124:1133-41. doi: 10.1385/abab:124:1-3:1133.

Abstract

The effects of ammonia fiber explosion (AFEX) pretreatment of switch grass using its major process variables are reported. The optimal pretreatment conditions for switchgrass were found to be near 100 degrees C reactor temperature, and ammonia loading of 1:1 kg of ammonia: kg of dry matter with 80% moisture content (dry weight basis [dwb]) at 5 min residence time. Hydrolysis results of AFEX-treated and untreated samples showed 93% vs 16% glucan conversion, respectively. The ethanol yield of optimized AFEX-treated switchgrass was measured to be about 0.2 g ethanol/g dry biomass, which is 2.5 times more than that of the untreated sample.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Ammonia / chemistry*
  • Cell Culture Techniques / methods*
  • Cellulase / chemistry*
  • Cellulose / chemistry
  • Cellulose / metabolism
  • Culture Media / chemistry*
  • Culture Media / metabolism
  • Energy-Generating Resources
  • Ethanol / metabolism*
  • Hydrolysis
  • Lignin / chemistry
  • Lignin / metabolism
  • Plant Components, Aerial / chemistry
  • Plant Components, Aerial / microbiology
  • Poaceae / chemistry*
  • Poaceae / microbiology*
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae / metabolism*
  • Steam
  • Water / chemistry

Substances

  • Culture Media
  • Steam
  • Water
  • lignocellulose
  • Ethanol
  • Ammonia
  • Cellulose
  • Lignin
  • Cellulase