Abstract
Cyclohexanone monooxygenase (CHMO) catalyzing Baeyer-Villiger oxidation converts cyclic ketones into optically pure lactones, which have been used as building blocks in organic synthesis. A recombinant Escherichia coli BL21(DE3)/pMM4 expressing CHMO originated from Acinetobacter sp. NCIB 9871 was used to produce epsilon-caprolactone through a simultaneous biocatalyst production and Baeyer-Villiger oxidation (SPO) process. A fed-batch process was designed to obtain high cell density for improving production of epsilon-caprolactone. The fed-batch SPO process gave the best results, 10.2 g/L of epsilon-caprolactone and 0.34 g/(L.h) of productivity, corresponding to a 10.5- and 3.4-fold enhancement compared with those of the batch SPO, respectively.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Acinetobacter / enzymology*
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Acinetobacter / genetics
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Bioreactors / microbiology
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Biotransformation
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Caproates / isolation & purification
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Caproates / metabolism*
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Catalysis
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Cell Culture Techniques / methods
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Cell Proliferation
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Cyclohexanones / metabolism*
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Escherichia coli / genetics
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Escherichia coli / growth & development*
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Escherichia coli / metabolism*
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Lactones / isolation & purification
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Lactones / metabolism*
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Oxidation-Reduction
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Oxygenases / genetics
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Oxygenases / metabolism*
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Protein Engineering / methods*
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Recombinant Proteins / metabolism
Substances
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Caproates
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Cyclohexanones
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Lactones
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Recombinant Proteins
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caprolactone
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cyclohexanone
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Oxygenases
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cyclohexanone oxygenase