Abstract
It is shown here by flow cytometry that Bacillus subtilis YabA negatively regulates the timing of replication initiation. When the level of YabA was reduced, replication began at a decreased cell mass and when the level was increased, initiation was delayed. Synchrony of replication initiation was also disrupted at low levels of YabA. Yfp-YabA localized as foci in cells. Since YabA was reported to interact with DnaN (beta subunit of DNA polymerase III), co-localization of Yfp-YabA with the polymerase was examined using a Cfp fusion with DnaX (tau subunit of DNA polymerase III). It is reported that YabA appears to localize at the replication forks only at a late stage of DNA replication.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacillus subtilis / genetics
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Bacillus subtilis / physiology*
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Bacterial Proteins / metabolism
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DNA Helicases / genetics*
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DNA Helicases / metabolism
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DNA Polymerase III / metabolism
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DNA Replication*
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DNA, Bacterial / biosynthesis*
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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DNA-Directed DNA Polymerase / metabolism
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Flow Cytometry
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Trans-Activators / genetics*
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Trans-Activators / metabolism
Substances
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Bacterial Proteins
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DNA, Bacterial
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DNA-Binding Proteins
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DnaX protein, Bacteria
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Trans-Activators
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replication initiator protein
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DNA Polymerase III
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DNA-Directed DNA Polymerase
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dnaN protein, Bacteria
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DNA Helicases