[L-fucose-specific lectin from pike perch (Lucioperca lucioperca L.) roe. Purification and studies of carbohydrate specificity]

Ukr Biokhim Zh (1999). 2004 Mar-Apr;76(2):75-9.
[Article in Ukrainian]

Abstract

L-fucose-specific lectin was purified from pike perch (Lucioperca lucioperca L.) roe by affinity chromatography on ovariomucine H-sepharose from a human ovary cyst. Three bands were detected after disk-electrophoresis in PAAG in alkaline (pH 8.9) and five bands--in acidic system (pH 4.3). According to electrophoresis data in 15% SDS-PAGE the lectin contains two components with molecular weight 13-14 kDa. Molecular weight of the lectin is 50 kDa according to gel-chromatography on tojopearl HW-55. The immunochemical properties of the lectins from perch (Persa fluviatilis L.) roe and pike perch (Lucioperca lucioperca L.) roe are similar.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Carbohydrates / analysis*
  • Chromatography, Gel
  • Electrophoresis, Polyacrylamide Gel
  • Lectins / isolation & purification*
  • Lectins / metabolism
  • Molecular Weight
  • Ovum / metabolism*
  • Perciformes / metabolism*
  • Substrate Specificity

Substances

  • Carbohydrates
  • Lectins
  • fucose-binding lectin